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If you want to print more than one copy of a document, use the -#num option, where num is replaced by the number of copies you want. For example, to print five copies of a file, use:

6.5.1 Register Direct Addressing In the register direct mode, the eight data registers (DGD7) or seven address registers (AO-A6) contain the data operand. For example, consider M0VE.W AO, D1. The source operand of this instruction is in addres register direct mode while the destination operand is in data register direct mode. Note that instructions with two operands have two addressing modes.

A feature in the ksh93 shell that s not available in the bash shell is the typeset command. This command allows you to set attributes for variables and functions. One of the attributes you can set is the data type that the variable uses. Table 22-3 lists the parameters used with the typeset command. The typeset command is a great way to convert numbers between different bases:

Perform high-speed, high-volume, numerical computations. Provide fast, accurate, and inexpensive communication within and between

2. Generate a 1024-bit RSA private key and save it to a file:

new or modi ed data, while the majority of the le remains unchanged and doesn t need to be backed up again.

km * moi'

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3. Repeat the previous steps to add user accounts for all users from Windows workstations on your LAN that you want to give access to your Linux system. 4. Type the following command to create the Samba password file (smbpasswd) on Fedora Linux systems:

Glycerol 3-phosphate dehydrogenase (mi tochondrial)

The optional SQL Server logins are useful when Windows authentication is inappropriate or unavailable. It s provided for backward compatibility and for legacy applications that are hard-coded to a SQL Server login.

Figure 3-18

1 5 7

dif cult task due to the enormous complexity of the NPC and the multiplicity of interactions among its components Until very recently, cryo-electron microscopy remained perhaps the only technique capable of providing detailed structural information on NPCs (137, 138) Rapid development of proteomic techniques, as well as the central role of MS in proteomics are certain to put MS at the center stage of investigations of NPC structure and function Indeed, MS has already begun to experience an enormous surge in popularity in studies related to structure, dynamic behavior, and mechanistic aspects of NPC function Burlingame and co-workers developed a strategy to identify proteins that interact with individual components of the nucleocytoplasmic transport machinery (139, 140) Further studies by this group provided a more detailed account as far as how these interactions actually occur (141).

The novel interactions among the components of nuclear pore traf cking machinery uncovered in this study will certainly be invaluable for understanding the mechanisms of nucleocytoplasmic transport The complicated structure of the NPC was initially believed to function as an elaborate mechanochemically gated portal, which promotes a series of binding and exchange reactions An alternative view of macromolecular transport through the NPC has recently been introduced based on the results of a series of studies in which MS has enjoyed a very prominent role (142, 143) Cataloging all protein components of a NPC using proteomic tools and MS allowed all proteins present in a highly enriched NPC fraction to be identi ed Localization of all identi ed Nups within the NPC using other (non-MS) biophysical and biochemical methods allowed a detailed architectural map of the yeast NPC to be produced (Figure 1121) (142).

This study eventually led to the introduction of a virtual gating model of NPC functioning (144) The virtual gating model explains the mechanism of rapid and selective macromolecular traf cking through the nuclear pore by invoking the notion of passive diffusion that can be enhanced by favorable interaction of the cargo-carrying Kaps with Nups When a macromolecule is con ned to a volume encompassed by the central channel of the NPC, its mobility is limited, leading to an entropy decrease Such entropic price of passage through the NPC increases as the macromolecular size increases, diminishing the probability of the passage and making the channel effectively impermeable to macromolecules above a certain size The densely packed Nups add to this entropic price, by further constraining the free space available for diffusion.

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